Random amplified polymorphic DNA (RAPD) analysis ofMycobacterium tuberculosisstrains in India
نویسندگان
چکیده
منابع مشابه
Random Amplified Polymorphic DNA (RAPD) Markers
Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplificatio...
متن کاملRandom amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India
The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served...
متن کاملUtility of Random Amplified Polymorphic DNA (RAPD) In Forensic Entomology
Forensic entomology is the study of insects found around the crime scene and are helpful for the estimation of time elapsed since death of a person i.e., post mortem interval (PMI). There are number techniques available for the identification of insect species and Random Amplified Polymorphic DNA (RAPD) is one of those. RAPD is a useful technique designed for the detection of sequence polymorph...
متن کاملRandom amplified polymorphic DNA (RAPD) fingerprinting of Mycoplasma gallisepticum isolates from chickens
Ten field isolates of Mycoplasma gallisepticum (MG) from different geographical areas of Iran were analyzed by random amplification of polymorphic DNA (RAPD). RAPD analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. MG isolates compared with reference strains (S6 and Mg SS) and vaccine strain (ts-11) and demonstrated...
متن کاملRandom amplified polymorphic DNA (RAPD) markers and its applications
In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD is a modification of the PCR in which a single, short and arbitrary oligonucleotide primer, able to anneal and prime at multiple locations throughout the genome, can produce a spectrum of a...
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ژورنال
عنوان ژورنال: Journal of Veterinary Science
سال: 2006
ISSN: 1229-845X
DOI: 10.4142/jvs.2006.7.2.181